Confocal Laser Scanning Microscopy and Multi Photon

Web Overviews

Concepts in Confocal Microscopy

An extensive collection of articles covering fundamentals, instrumentations, applications, and terminology. From leading experts in confocal microscopy including Michael W. Davidson and Olympus America Inc.

Introduction to Confocal Microscopy

From basic concepts to advance articles on aberrations, three-color imaging, and confocal reflection microscopy from leading experts. From Nikon MicroscopyU and Olympus America's Optical Microscopy for Biomedical Applications

Laser Scanning Confocal Microscopy

Molecular Expressions' articles including those done in conjunction with Nikon MicroscopyU and Olympus America Inc.

A Visual Slice of the Cellular World

Nice introduction from Nana Rezai, BioTeach, University of British Columbia.

PDF Overviews

Light Microscopy Techniques for Live Cell Imaging

David J. Stephens and Victoria J. Allan, Science 2003 300: 82-86

Basic Concepts in Laser Scanning Confocal Microscopy

Highly recommended resource. From Nathan S. Claxton, Thomas J. Fellers, and Michael W. Davidson, National High Magnetic Field Laboratory, The Florida State University.

Instrumentation and Techniques

In addition to the outstanding resources at Molecular Expression Laser Scanning Confocal Microscopy, Nikon MicroscopyU and Olympus America's Optical Microscopy for Biomedical Applications the following resources are of interest.

"Memoir on Inventing the Confocal Scanning Microscope"

Marvin Minsky, Published in Scanning, vol.10 pp128-138, 1988

Technical Information from Omega Optical

Includes articles, gallery of images, application notes and more.

Laser Scanning Confocal Applications

Microscopy, Image Analysis and 3D Reconstruction

Purdue University CD-ROM series, James B. Pawley and J. Paul Robinson, Eds.
The cd-rom, originally distributed at no cost at professional meetings, is now online. The online version contains a large collection of laser confocal microscopy images, a series of power point presentation, references, and time lapse movies.

Technical Notes and Applications References

Application Notes include a wide and varied range of applications of confocal and 2 photon microscopy. The Technical Notes are geared toward optimizing results with the Bio-Rad system. The bibliography database has references to over 6000 articles in confocal microscopy. From Bio-Rad.

Looking Inside Cells and Tissues by Optical Sectioning with a Confocal Laser Scanning Microscope

From Bones to Atoms: Imaging nature across dimensions. M.E. Müller Institute for Microscopy, Biozentrum, University of Basel

Fishscope, University of Washington

Fishscope is a series of time lapse recordings of the development of zebra fish from early cleavage through the formation of organ rudiments.

Multi Photon

Multiple-photon excitation fluorescence microscopy

An introduction from LOCI: Laboratory for Optical and Computational Instrumentation, University of Wisconsin Madison

List of More Internet Resources

Confocal Microscopy on the WWW

University of Arizona College of Pharmacy

Laser Scanning Confocal Facilities

Listservers and Newsgroups

Microscopy News Groups