Learn about the science underlying public health issues by perusing -- and using -- digitized images on view.
This well done site includes principles of virus structure, Electron Microscopy:How images are created by negative staining, electron microscopy images, and lecture notes.
George E. Palade – Nobel Lecture, December 12, 1974
John Cowley made important theoretical, experimental, and instrumentation development contributions to high resolution electron microscopy, scanning transmission electron microscopy, and the use of electron scattering for structure analysis. He had an extraordinary ability to explain processes and his book Diffraction Physics remains the authoritative work on techniques of scattering electrons, X-rays, or neutrons.
In the early 1950s, Palade’s utilization of the then new technologies, such as electron microscopy and ultracentrifugation,brought understanding of the cell to a new level.
This autobiography/biography was written at the time of the award and later published in the book series Les Prix Nobel/Nobel Lectures.
National Inventors Hall of Fame
James Hillier and Albert Prebus designed and built the first successful electron microscope while graduate students at the University of Toronto. He went on to work at RCA and developed techniques that extended the application of the electron microscope where he invented the electron microprobe. The site includes a short biography and a complete listing of the publications.
Working in Albert Claude research group at Rockefeller University, George Palade developed the sucrose method for cell fractionation which isolated organelles without distorting their shapes. By using osmium tetroxide better contrast was obtained in electron microscopy and Palade was able to describe the fine structure of mitochondria. This summary of Palade's work at Rockefeller University include a link references to journal articles.
Albert Claude pioneered techniques of cell fractionation and was the first to use an electron microscopes to study cells. This first image of an intact cell, a collaboration with Keith Portor and Ernest Fullam, was termed it the "birth certificate" of the field of cell biology by George Palade. This summary of his work at Rockefeller University includes links to selected publications.
Pratibha Gai, the 2013 L'Oreal-Unesco Women In Science European Laureate discusses the importance of encouraging women in science and her breakthrough in modifying a transmission electron microscope so thatgas-solid reactions could be observed at the atom level.
The George Pallade collection includes early electron micrographs from Rockefeller University and Yale University. Many of the images are significant for their contribution to our understanding of cellular structure and processes. The images were used in the cell biology courses at Yale University by Drs. George Palade and Marilyn Farquhar. It was Dr. Palade's wish that the collection of images be freely available to students and scientists world-wide.
Images from scanning electron microscopes, transmission electron microscope images, DualBeam instruments, and focussed ion beam. The site includes images taken by users of FEI instruments and by FEI. the images are organized by instrument and by area of interest.
The first electron microscope image of an intact cell at a magnification of 1600x. The image reveals mitochondria, the Golgi apparatus and a "lace-like reticulum" which was later named the "endoplasmic reticulum" by Keith Porter. The work was a collaboration between Albert Claude and Keith Porter from the Rockefeller Institute for Medical Research and Earnest Fullam of the Interchemical Corporation. the original publication is available in online.
Introduction to the Transmission Electron Microscope.
The transmission electron microscope uses principles that originated in light microscopy and which therefore rely on the properties of waves. These principles are illustrated.
Brief introduction to the Principle and application of Imaging electron energy loss spectroscopy
Introduction to the Dynamic Transmission Electron Microscope (DTEM) at Lawrence Livermore Laboratories that provides the ability to image transient behaviors such as how a chemical reaction, structural deformation or phase transformation takes place with an unprecedented combination of spatial and temporal resolution: nanometers and nanoseconds.
For biological and material scientists who are new to TEM, but need to learn to use the technique. Includes alignment instructions.
Current course notes for electron microscopy in pdf and PowerPoint. The Bottom Line is especially helpful and focuses basic concepts that are important for understanding the fundamental principles of transmission electron microscopy, biological specimen preparation, and three-dimensional image processing and reconstruction. Winter 2011 course is 3D Electron Microscopy of Macromolecules.
Complete course notes.
This is a set of resources designed to accompany an introductory course on transmission electron microscopy. The level is appropriate for students with an understanding of some elementary physics.
Learn to use a transmission electron microscope...fast. This site has put together by John Rodenburg, who has been teaching graduate students to use TEMs for over twenty years (at the University of Cambridge, Sheffield Hallam and Sheffield Universities). Now includes a new section on TEM/STEM alignment.
Cource notes, protocols, and power point presentation for specimen preparation for scanning electron and transmission electon microscopy and overviews of instrumentation.
A section on focussed ion beam instruments is included in the FEI booklet "An Introduction to Electron Microscopy". The booklet is an excellent overview of of electron microscopy and nanotechnology for students and teachers. It is available as a pdf for download.
Fluctuation microscopy has been used to study disordered materials including amorphous Ge and silicon. This paper is a general introduction to the technique.
The Royal Swedish Academy of Sciences has decided to award the 1986 Nobel Prize in Physics by one half to Professor. Ernst Ruska, Fritz-Haber-Institut der Max-Planck-Gesellschaft, Berlin, Federal Republic of Germany, for his fundamental work in electron optics, and for the design of the first electron microscope and the other half, jointly to Dr Gerd Binnig and Dr Heinrich Rohrer, IBM Research Laboratory, Zurich, Switzerland, for their design of the scanning tunnelling microscope.
From Nobel Lectures, Physics 1981-1990, Editor-in-Charge Tore Frängsmyr, Editor Gösta Ekspång, World Scientific Publishing Co., Singapore, 1993
History of electron microscopy at Rockefeller University and the
Peer reviewed article describing using diffractive imaging to improve resolution in a scanning electron microscope by an order of five. The method called electron ptychography is a lenless method that reconstructs the image from the complex electron waves that have passed trought the sample.